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. 2023 Jan 1;19(1):89–103. doi: 10.7150/ijbs.74227

Figure 5.

Figure 5

Overexpression of MDK accounted for miR-1275 function in BC. A. The mRNA and protein expression of MDK was measured by RT-qPCR and western blot. B-D. Drug resistance of MCF-7 and MCF-7/ADR transfected with miR-NC, miR-mimics, miR-knocked-out, miR-mimics and MDK plasmid, miR-knocked-out and MDK siRNAs or miR-knocked-out and MDK antibody were determined by CCK-8 assays (B.), flow cytometry analysis (C.), and colony formation experiments (D.). E-F CSC properties of MCF-7 and MCF-7/ADR transfected with miR-NC, miR-mimics, miR-knocked-out, miR-mimics and MDK plasmid, miR-knocked-out and MDK siRNAs or miR-knocked-out and MDK antibody were determined by flow cytometry analysis (E.) and mammosphere formation assay (F.). G. CD44, CD133, NANOG, OCT4, SOX2 and BMI1 expression was measured by western blot in MCF-7 and MCF-7/ADR treated with miR-NC, miR-mimics, miR-knocked-out, miR-mimics and MDK plasmid or miR-knocked-out and MDK siRNAs. *P < 0.05, **P<0.01, ***P < 0.001. The data expressed as the mean ± SD. Scale bar, 100 µm.