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. 2023 Jan 1;19(1):89–103. doi: 10.7150/ijbs.74227

Figure 6.

Figure 6

The reduction of miR-1275 promotes BC cells chemoresistance via MDK/AKT axis. A. MDK, PI3K, P-PI3K, AKT and P-AKT expression was measured by western blot in MCF-7 and MCF-7/ADR. B-D. Drug resistance of MCF-7 and MCF-7/ADR transfected with miR-NC, miR-mimics, miR-knocked-out, miR-mimics and 740Y-P, miR-knocked-out and iMDK or miR-knocked-out and LY294002 were determined by CCK-8 assays (B.), flow cytometry analysis (C.), and colony formation experiments (D.). E-F. CSC properties of MCF-7 and MCF-7/ADR transfected with miR-NC, miR-mimics, miR-knocked-out, miR-mimics and 740Y-P, miR-knocked-out and iMDK or miR-knocked-out and LY294002 were determined by flow cytometry analysis (E.) and mammosphere formation assay (F). G. CD44, CD133, NANOG, OCT4, SOX2 and BMI1 expression was measured by western blot in MCF-7 and MCF-7/ADR treated with miR-NC, miR-mimics, miR-knocked-out, miR-mimics and 740Y-P, miR-knocked-out and iMDK or miR-knocked-out and LY294002. *P < 0.05, **P<0.01, ***P < 0.001. The data expressed as the mean ± SD. Scale bar, 200 µm.