Figure 1.

Blockade of TREM-1 reduced intrapulmonary inflammation and limited glycolysis in LPS-induced ALI mice. C57BL/6J mice were intravenously injected with LR12 (5 mg/kg) 2 h before the LPS administration (5 mg/kg, i.t.). (A) Six hours later, mouse lungs were excised, and lung histopathology was performed with H&E staining. One representative picture of six mice in each group is shown. (B) Inflammation score was measured, n=6 mice/group. (C) Pro-IL-1β, IL-1β p17, and iNOS in the lung lysates were assessed by western blot with β-actin as a loading control. (D-F) The western blot results were quantitated using Image Lab, n=8 mice per group. (G-H) Lactate concentration in BALF and serum was assayed, n=4-9 mice/group. (I) Expression of Hk2, Pfkfb3, and Hif-1α mRNA in the lungs was detected by real-time PCR. Data was normalized to housekeeping gene β-actin, n=5-10 mice/group. (J) Glycolysis-associated proteins of HK2, p-mTOR, mTOR, and HIF-1α in the lung lysates were assessed by western blot with α-tubulin as a loading control. (K-N) Quantification of indicated protein levels in (J), n=6-8 mice/group. In all cases, the experiment was repeated twice. Dots represent individual animal values. Statistical analysis was performed using One-way ANOVA adjusted by Tukey's multiple comparison test for Control vs. ALI or ALI vs. LR12+ALI. Error bars indicate mean ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001. Original western blots represented in graphs are available in Figure 1-source data.