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. 2023 Jan 1;19(1):242–257. doi: 10.7150/ijbs.77304

Figure 3.

Figure 3

TREM-1 triggered the NLRP3 inflammasome activation in macrophages. Macrophages were incubated with plate-bound isotype-matched control or plate-bound anti-TREM-1 mAb (10 μg/mL). (A-C) Six hours later, Nlrp3, Pro-caspase-1, and Pro-il-1β mRNA expressions in macrophages were measured using qPCR. Data was normalized to housekeeping gene β-actin, n=3. (D) Twenty-four hours later, protein expression of NLRP3, pro-caspase-1, and pro-IL-1β in the cell lysate was detected by western blot with α-tubulin as a loading control. (E-G) Quantification of indicated protein levels in (D), n=3. (H-J) Caspase-1 p10 and IL-1β p17 in the supernatant were detected by western blot, n=3. (K) IL-1β contents in the supernatants were analyzed with ELISA, n=5. n represents experiments performed on different macrophages from separate mice. Bar graphs represent mean ± SD. Student's t-test (two-tailed, unpaired) was used to compare Control and Mab1187: * P < 0.05, ** P < 0.01, and *** P < 0.001.