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. 2023 Jan 1;19(1):242–257. doi: 10.7150/ijbs.77304

Figure 8.

Figure 8

TREM-1 triggered the NLRP3 inflammasome activation by enhancing mitochondrial fission in macrophages. Macrophages were stimulated with 10 μg/mL of an agonist anti-TREM-1 mAb. (A-B) Western blot and quantification of p-DRP1ser616 and DRP1 in control or 24 h TREM-1-activated macrophages. n=3 biological replicates. Statistical analysis was performed using Student's t-test (two-tailed, unpaired). Macrophages were premixed with PBS control or Mdivi-1 (100 nM) before incubating with plate-bound agonistic anti-TREM-1 mAb for 24 h. (C) Protein levels of NLRP3, pro-IL-1β, IL-1β p17, and caspase-1 p10 in macrophages were detected by western blot. (D-G) Quantification of NLRP3, pro-IL-1β, IL-1β p17, and caspase-1 p10 (C), n=3 biological replicates. Statistical analysis was performed using One-way ANOVA. Data were expressed as the mean ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001.