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. 2023 Jan 1;19(1):34–49. doi: 10.7150/ijbs.72381

Figure 3.

Figure 3

The knockdown of KMT2B expression impairs CC migration, invasion and angiogenesis in vitro. (A, B) The protein levels of KMT2B in HeLa and C-33A cells after knockdown by CRISPR/Cas9 method. The histogram shows the densitometric analysis of the bands. We selected the first sgRNA sequence with the highest knockdown efficiency for subsequent functional experiments. (C) KMT2B-knockdown and control HeLa or C-33A cells were cultured in non-coated chambers for 8 h. The cells that migrated through the chamber were stained using crystal violet and photographed. Quantitated the cells migration by counting the migrated cells. (D) KMT2B-knockdown and control HeLa or C-33A cells were cultured in Matrigel-coated chambers for 12 h. The cells that invaded through the Matrigel were stained using crystal violet and photographed. Quantitated the cells invasion by counting the invaded cells. (E) Images of HUVECs tube formation with incubating in KMT2B-knockdown and control HeLa or C-33A cells condition medium. Quantitated total length of tubes by Image Pro Plus software after incubating for 4 h in KMT2B-knockdown and control HeLa or C-33A groups. Data represents mean ± SD (n = 3). All the experiments have been repeated at least once with similar results. Statistical significance was determined by students t test. *, p < 0.05 **, p < 0.01; ***, p < 0.001.