FIG. 3.

Adhesion of A. fumigatus conidia to native and deglycosylated fibronectin. Fibronectin was treated with glycosidases (shaded bars) or no enzymes (open bars) under denaturing (3 h at 37°C following heating to 100°C for 5 min in denaturation buffer) or nondenaturing (6 h at 37°C) conditions, and then microtiter plates were coated with it. The background wells contained BSA only (solid bars). Peroxidase-labeled conidia were added to the wells, and the number of bound conidia was determined by measuring the optical density (OD) at 490 nm after the addition of substrate. The values are the means ± standard deviations of triplicate wells, and the figures are representative of three independent experiments. ∗, P < 0.05 versus nondenatured.