FIG. 5.

Effects of negatively charged carbohydrates on the adhesion of A. fumigatus conidia to fibronectin and basal lamina. Peroxidase-labeled conidia were added to fibronectin-coated microtiter plates (A) or to basal-lamina-coated microtiter plates (B) in the presence of heparin (Hep), dextran sulfate (DS), keratan sulfate (KS), or chondroitin sulfate (CS). Unbound conidia were removed by washing the plates with PBS–0.05% Tween 20, and the number of bound conidia was determined by measuring the optical density (OD) at 490 nm. Background wells contained BSA only. The values are the means ± standard deviations of triplicate wells. These figures are representative of two independent experiments. ∗, P < 0.05 versus none.