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. 2022 Dec 9;59:102573. doi: 10.1016/j.redox.2022.102573

Fig. 10.

Fig. 10

LPO co-localization with XOR. (A–B) XOR activity of gently prepared tissue lysate were assayed within 2h from the start of tissue homogenization. XOR activity was assayed as described in Fig. 9. LPO KO; LPO knock out mouse, GD; gestational day. Results are expressed as the mean ± SD (n = 5 per group). (A) The total (NAD + plus O2-dependent) urate formation (green) and O2-dependent urate formation (red: XO activity). (B) XO content was indicated by the percentage of O2-dependent urate formation to total urate formation. (C–D) Western blot analysis was performed in a 5–20% polyacrylamide gel. Lane M, molecular mass standards (Precision Protein Standards, Bio-Rad Laboratories); lane S, salivary gland. Each lane contains 10 μg of protein. (C) XOR (shown as arrow) was detected with anti-rat XOR antibody. (D) LPO (shown as arrow) was detected with anti-mouse LPO antibody. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)