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Primers and probes used in the PMAxx-qPCR assay for detecting the P72 gene of ASFV.
| Gene | Name | Sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| P72 | #1-Forward | TCCTGAAAGCTTATCTCTGCG | 75 |
| #1-Reverse | AGATTGGCACAAGTTCGGAC | ||
| #1-Probe | FAM-TGAGTGGGCTGCATAATGGCGTT-BHQ | ||
| P72 | #2-Forward | AAGGTAATCATCATCGCACC | 163 |
| #2-Reverse | ATCCGATCACATTACCTATTAT | ||
| #2-Probe | FAM-TCCGTAACTGCTCATGGTATCAATCTT-BHQ | ||
| P72 | #3-Forward | TTGATACCATGAGCAGTTACGG | 189 |
| #3-Reverse | AGATTGGCACAAGTTCGGAC | ||
| #3-Probe | FAM-TGAGTGGGCTGCATAATGGCGTT-BHQ |
