FIG. 2.

Stimulation of 4E6 T-cell hybridoma with the native 62-kDa component of SEA and rSm-PEPCK. Hybridoma cells were cultured with the indicated antigens in the presence of APC, and their stimulation was assessed by incorporation of [³H]dThd into HT-2 indicator cells as described in Materials and Methods. Data are expressed as mean counts per minute (error bars, 1 standard deviation). Background counts per minute from hybridoma cells cultured in the absence of antigen were subtracted. Also shown for comparison are the responses to rSm-p40 and SEA. This experiment was repeated three times with similar results.