FIG. 5.

Cytokine production by CD4⁺ Th cells from BL/6, CBA, and BALB/c mice stimulated with rSm-PEPCK. CD4⁺ Th cells were isolated from mesenteric lymph nodes of 8.5-week-infected mice and cultured with the indicated antigens in the presence of APC as described in Materials and Methods. The cytokines were measured in culture supernatants by enzyme-linked immunosorbent assay. Data are expressed as means of triplicate determinations (error bars, 1 standard deviation). Also shown for comparison are responses to rSm-p40 and SEA. This experiment was repeated three times for BL/6 and CBA and twice for BALB/c, with similar results.