FIG. 7.

4E6 T-cell hybridoma responses to synthetic peptides. Hybridoma cells were cultured with the indicated peptides in the presence of APC, and their stimulation was assessed by incorporation of [³H]dThd into HT-2 indicator cells as described in Materials and Methods. Data are expressed as mean counts per minute (error bar, 1 standard deviation). Background counts per minute from hybridoma cells cultured in the absence of antigen were subtracted. Also shown for comparison are responses to rSm-PEPCK and SEA. The peptides were tested at least four times with similar results. (A) First set of peptides; (B) second set of peptides; (C) control antigens.