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. 2022 Dec 5;13:1048695. doi: 10.3389/fpls.2022.1048695

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Copyright © 2022 Miroshnichenko, Timerbaev, Klementyeva, Pushin, Sidorova, Litvinov, Nazarova, Shulga, Divashuk, Karlov, Salina and Dolgov

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the genome-edited wheat line carrying the 8 bp deletion in the promoter region of vrn-A1. (A) Effect of vernalization on the heading date of wheat plants carrying the 8 bp deletion (GE31-10-19 d8) in comparison with the ‘Chinese Spring’ plants and transgene-free null-segregant plants (WT31-10). NS: nonsignificant. (B) Relative expression of the vrn-1A gene in wheat plants carrying the 8 bp deletion in the promoter region of vrn-A1 (GE31-10-19 d8) in comparison with the ‘Chinese Spring’ (CS) plants; Tukey’s multiple comparisons test, ***p < 0.001; ns: nonsignificant.