Figure 5.

Direct detection of ssDNAs in lysates from vector-transfected cells. Mouse FL-10 cells were transfected with the indicated vectors, and 24 h later the cells were lysed for isolation of low molecular weight DNA. The samples were analyzed for the presence of the predicted ssDNA products, either AG34 or rev34, by primer extension with 15 nt primers 5′ end-labeled with ³²P. Lanes 1 and 2 contain 5′ end-labeled synthetic oligonucleotides of length 30 and 41 as size standards to evaluate the sizes of the primer extension products. For lanes 3–7, the primer complementary to AG34 was used. For lane 8, the primer complementary to rev34 was used. Note that lanes 1 and 2 present oligonucleotide size standards only; these lanes are not intended to provide any information as to quantity or yield, and so the band intensities are not meaningful.