Table 1.

Chlorophyll fluorescence parameters, enzymatic activity, malondialdehyde levels, and photosynthetic pigments content measured on the last day of the experimental period in fully expanded leaves of M. maximus developed at two levels of [CO₂] and temperature.

Parameter	Treatments				ANOVA
	aCaT	eCaT	aCeT	eCeT	C	T	CxT
ETR	36.3 ± 3.3	51.5 ± 3.4	47.7 ± 4.9	58.3 ± 4.6	*	*	ns
Y(NPQ)	0.95 ± 0.05	0.87 ± 0.05	0.85 ± 0.11	0.75 ± 0.02	ns	ns	ns
qL	0.21 ± 0.03	0.26 ± 0.02	0.20 ± 0.03	0.29 ± 0.009	**	ns	ns
qP	0.29 ± 0.03	0.36 ± 0.02	0.30 ± 0.03	0.41 ± 0.011	**	ns	ns
Y(II)	0.10 ± 0.009	0.14 ± 0.010	0.13 ± 0.013	0.17 ± 0.006	**	*	ns
Y(NO)	0.19 ± 0.004	0.19 ± 0.009	0.21 ± 0.017	0.21 ± 0.004	ns	ns	ns
CAT	30.1 ± 4.9	31.5 ± 1.6	29.8 ± 2.7	29.1 ± 4.4	ns	ns	ns
APX	0.32 ± 0.03	0.36 ± 0.04	0.26 ± 0.03	0.29 ± 0.05	ns	*	ns
POD	1.7 ± 0.17	2 ± 0.22	1.4 ± 0.04	1.7 ± 0.17	ns	ns	ns
SOD	30.8 ± 0.9	29.4 ± 3.9	25.7 ± 2.1	27.6 ± 3.5	ns	ns	ns
MDA	259.4 ± 32.8	283.8 ± 21.6	295.5 ± 17.2	283.2 ± 14.6	ns	ns	ns
Chl a	5.3 ± 0.4	4.5 ± 0.3	4.8 ± 0.2	5.2 ± 0.3	ns	ns	*
Chl b	1.9 ± 0.1	1.6 ± 0.1	1.7 ± 0.1	1.8 ± 0.1	ns	ns	*
Carot	2.8 ± 0.2	2.5 ± 0.1	2.6 ± 0.1	2.6 ± 0.1	ns	ns	ns
Chl a+b	7.2 ± 0.5	6.2 ± 0.4	6.6 ± 0.3	7.3 ± 0.2	ns	ns	*
Chl a/b	2.8 ± 0.07	2.7 ± 0.05	2.8 ± 0.03	2.7 ± 0.05	ns	ns	ns
Chl/Carot	2.6 ± 0.03	2.5 ± 0.01	2.6 ± 0.04	2.5 ± 0.02	ns	ns	ns

ETR , electron transport rate; Y(NPQ) , quantum yield of regulated energy dissipation; qL , photochemical quenching (lake model); qP , photochemical quenching (puddle mode); Y(II) , effective PS II quantum yield during illumination; Y(NO) , quantum yield of non-regulated energy dissipation; CAT , catalase activity; APX , ascorbate peroxidase activity; POD , non-specific superoxide activity; SOD , superoxide dismutase activity; MDA , leaf malondialdehyde level; Chl a , Chlorophyll a concentration; Chl b , Chlorophyll b concentration; Carot , carotenoid concentration. ANOVA results are shown for each parameter, C, [CO₂] effect; T, temperature effect; C×T, interaction between C and T. *P < 0.05, **P < 0.01, ***P < 0.001, ns, non-significant. Fluorescence parameters were analyzed at 784 μmol photons m⁻² s⁻¹.