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. 2022 Dec 5;13:1022228. doi: 10.3389/fimmu.2022.1022228

Table 2.

The expression level of the PD-1/PD-L1 in animal models.

First author PD-1/PD-L1 Animal Exposure Sample Cell types Method Expression level Ref.
Youliang Zhao PD-1 6-8 weeks male C57BL/6 mice Silica Lung / WB
IHC
FCM
PCR
Upregulation (28)
PD-L1 Lung / Upregulation
Ye Lu PD-L1 4-6 weeks female C57BL/6 mice BLM Lung Fibroblasts WB
IHC
Upregulation (29)
Xia Guo PD-L1 12-16 weeks male C57BL/6 mice BLM Lung Fibroblasts IF Upregulation (17)
Dong Wang PD-1 8 weeks male C57BL/6 mice BLM Lung / IHC Upregulation (30)
Peripheral blood CD4+ T cells FCM Upregulation
Lu Cui PD-L1 11-12 weeks male C57BL/6 mice BLM Lung Fibroblasts Mass cytometry
IF
FCM
Upregulation (20)
JUN-induced lung fibrosis mice JUN Lung Fibroblasts Upregulation
IL-6 knock out mice and wildtype BLM Lung Fibroblasts Upregulation
NSG mice Primary human fibrotic lung fibroblasts engraftation Lung Fibroblasts Upregulation
PD-1 11-12 weeks male C57BL/6 mice BLM Lung CD8+T cells Mass cytometry Upregulation
Yan Geng PD-L1 6-8 weeks female NSG mice CD274high and CD274low IPF lung normal fibroblasts injection Lung Fibroblasts PCR
RNA-seq
Upregulation (21)
Ke Ni PD-L1 4-6 weeks Rag2-/-γc-/-mice BLM Lung MSC FCM Upregulation (22)
Lindsay J. Celada PD-1 7-9 weeks old C57BL/6J mice BLM Lung CD4+ T cells FCM Upregulation (25)

IPF, idiopathic pulmonary fibrosis; PD-1, programmed cell death 1; PD-L1, programmed death-ligand 1; IF, immunofluorescence; FCM, flow cytometry; IHC, immunohistochemistry; WB, western blot; PCR, polymerase chain reaction; BLM, bleomycin; NSG, NOD-SCID-IL2Rγc–/–; RNA-seq, RNA sequencing; MSC, mesenchymal stem cell.