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. 2022 Dec 5;13:1024039. doi: 10.3389/fimmu.2022.1024039

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Copyright © 2022 Maus, Leontovich, Moore, Fogarty, Guo, Davidson, Tekin, Atherton, Schimke, Dicke, Chen and Markovic

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cylic MxIF applied to whole excisional lymph node biopsies provides phenotypic and functional status of single cells in the tumor core. (A) Virtual H&E of whole excisional LN biopsy at 10X objective with pathologist-annotated tumor mass fields of view indicated (red boxes). (B) Virtual H&E of single field of view (FOV) at 20X objective. (C) MxIF overlay of the same FOV visualizing 9 cellular phenotypes with 12 antibodies. (D) Phenotypic classification overlay, filled colors indicate assigned cell class. (E) Inset from yellow outline in panel (D) indicating expression profile of individual cells. (F) Composite expression profile of all markers in the panel design for a single cell highlighted in Panel (E).