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. 2022 Dec 5;13:1024039. doi: 10.3389/fimmu.2022.1024039

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Copyright © 2022 Maus, Leontovich, Moore, Fogarty, Guo, Davidson, Tekin, Atherton, Schimke, Dicke, Chen and Markovic

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single cell quantification of tumor core regions is not sufficient to differentiate based on recurrence outcome. Abundance plots of 3-tier classification (tumor, immune and stromal cells) across all FOVs on a per-patient basis (A). Abundance plots of immune cell phenotypic classes (B) and abundance plots of TIL subsets based on PD1 expression (C). Distribution of patients displayed on box plot as green circles (no recurrence) and red triangles (recurrence).Acronyms are as follows B cells (B), Cytotoxic T cell (CTC), Dendritic cells (DC), Macrophages (MAC), Neutrophils (NEUT), Pan-T cells (Pan-T), Stromal cells (SC), T helper cells (THC), T regulatory cells (Treg). ns indicates no significance.