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. 2022 Nov;10(22):1218. doi: 10.21037/atm-22-5159

Figure 1.

Figure 1

Knockdown of BAG3 promotes inflammation in BV2 cells. (A,B) BV2 cells were transfected with NC siRNA or BAG3 siRNA for 24 h, and expression of BAG3 in BV2 cells was demonstrated by Western blotting and in statistical histograms. n=3; mean ± SEM; **P<0.01, vs. NC group. (C) BV2 cells were transfected with NC siRNA or BAG3 siRNA for 24 or 48 h, and expression of IL-1β, TNF-α, and IL-6 in BV2 cells was shown by RT-qPCR. n=3; mean ± SEM; **P<0.01, ***P<0.001, vs. Con group; ns, not significant. BAG3, Bcl2-associated athanogene 3; NC, normal control; si, short interfering; IL, interleukin; mRNA, messenger RNA; TNF, tumor necrosis factor; SEM, standard error of mean; RT-qPCR, reverse transcription quantitative polymerase chain reaction.