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. 2022 Nov;10(22):1218. doi: 10.21037/atm-22-5159

Figure 2.

Figure 2

BAG3 was involved in activation of the NLRP3 inflammasomes in microglia induced by LPS. (A,B) BV2 cells were incubated with LPS (1 µg/mL) for 24 h, and expression of BAG3 in BV2 cells was demonstrated by Western blotting and in statistical histograms. n=3; mean ± SEM; ***P<0.001 vs. PBS group. (C,D) BV2 cells were transfected with NC siRNA or BAG3 siRNA for 24 h and incubated with LPS (1 µg/mL) for 24 h. Expression of BAG3 and NLRP3 in BV2 cells was shown by Western blotting and expression of the NLRP3 was shown in statistical histograms. n=3; mean ± SEM; *P<0.05, **P<0.01, ***P<0.001. BAG3, Bcl2-associated athanogene 3; PBS, phosphate-buffered saline; LPS, lipopolysaccharide; NLRP3, NOD-like receptor family, and the pyrin domain-containing 3; NC, normal control; si, short interfering; SEM, standard error of mean.