Figure 5.

Overexpression of BAG3 enhances autophagy in an inflammatory environment. (A-F) BAG3-AAV or Con-AAV was injected into the bilateral striatum of C57BL/6 mice. After 21 days, LPS or PBS was injected into the bilateral striatum. At 3 days after LPS injection, expression of p62, Beclin1, BAG3, Atg5, and LC3 was demonstrated by western blotting and in statistical histograms. n=3; mean ± SEM; *P<0.05, **P<0.01, ***P<0.001; ns, not significant. LC3, light chain 3; BAG3, Bcl2-associated athanogene 3; AAV, adeno-associated virus; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SEM, standard error of mean.