Figure 6.

Overexpression of BAG3 reduces LPS-induced pyroptosis. (A-E) BAG3-AAV or Con-AAV was injected into the bilateral striatum of C57BL/6 mice. After 21 days, LPS or PBS was injected into the bilateral striatum. At 3 days after LPS injection, expression of NLRP3, BAG3, caspase-1, and cleaved capase-1 was demonstrated by Western blotting and in statistical histograms. n=3; mean ± SEM; *P<0.05, **P<0.01, ***P<0.001; ns, not significant. NLRP3, NOD-like receptor family, and the pyrin domain-containing 3; BAG3, Bcl2-associated athanogene 3; AAV, adeno-associated virus; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SEM, standard error of mean.