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. 2022 Nov;10(22):1216. doi: 10.21037/atm-22-5064

Figure 3.

Figure 3

ISO inhibits the proliferation, migration and invasion of SKOV3 and HO8910 cells in vitro. (A,B) Viability of SKOV3 and HO8910 cells treated with ISO (0–20 µM) for 24 h measured by CCK-8 assay. (C,D) Effect of ISO 15 µM treatment for 1–5 days on the viability of OC cells determined by CCK-8 assay. SKOV3 and HO8910 cells were treated with 15 µM ISO for 24 h. (E) Colony formation assay. Crystal violet staining; magnification, ×1. (F,G) Wound healing experiment and images of monolayers were captured under an optical light microscope. Scale bar =200 µm. (H) Transwell assay. Crystal violet staining; scale bar =200 µm. (I) Western blot detects the expression of Ki67, MMP9 and MMP2 protein. Compared with the Control group, *, P<0.05. ISO, isorhamnetin; OC, ovarian cancer; MMP, matrix metalloproteinase; OD, optical density.