Figure 5.

HIF1α promotes F-actin ring formation. (A) RAW264.7 cells were induced with RANKL with or without L-mimosine (0, 50, 100, and 150 µM) at the indicated concentrations for 5 d. Images of F-actin (red) and nuclei (blue) were acquired using a fluorescence microscope (magnification, 100×). (B,C) Quantification analyses of F-actin ring size and number of nuclei per osteoclast. The data are expressed as the mean ± SD of the 3 independent experiments; ^##, P<0.01, vs. control group; **, P<0.01 vs. RANKL group. HIF1α, hypoxia-inducible factor 1 alpha; RANKL, receptor activator of NF-kB ligand; L-MIM, L-mimosine; SD, standard deviation.