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. 2022 Nov;10(22):1227. doi: 10.21037/atm-22-4603

Figure 7.

Figure 7

HIF1α upregulates NFATc1, c-Fos, and c-Jun expression during osteoclast differentiation. (A) RANKL-stimulated RAW264.7 cells were treated with or without different concentrations of L-mimosine (0, 50, 100, and 150 µM). (H) RANKL-induced RAW264.7 cells were treated with both L-mimosine and BAY-87-2243, and proteins, including NFATc1, c-Fos, and c-Jun, were measured using western blotting. (E-G,L-N) The protein expression level was quantitatively analyzed relative to β-actin and calculated using ImageJ software. (B-D,I-K) Quantitative analyses of the relative mRNA expression of osteoclasts specific genes: transcription factors NFATc1, c-Fos, and c-Jun were performed by RT-PCR in the RANKL-induced RAW264.7 cells treated with L-mimosine and L-mimosine + BAY-87-2243. All the data in the figures are presented as the mean ± SD of the 3 independent experiments; #, P<0.05, ##, P<0.01 vs. control group; *, P<0.05, **, P<0.01 vs. RANKL group. HIF1α, hypoxia-inducible factor 1 alpha; RANKL, receptor activator of NF-kB ligand; L-MIM, L-mimosine; RT-PCR, real-time polymerase chain reaction; SD, standard deviation.