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. 2022 Nov;10(22):1227. doi: 10.21037/atm-22-4603

Figure 8.

Figure 8

HIF1α promotes the RANKL-activated MAPK pathway. RAW264.7 cells were pretreated with L-mimosine or both L-mimosine and BAY-87-2243 for 0.5 h, followed by 35 ng/mL of RANKL for the indicated times, and the total proteins were extracted and evaluated by western blot assays for the MAPK (A,E) pathways. The average ratios of phosphor-p38 relative to p38, phosphor-ERK relative to ERK, and phosphor-JNK relative to JNK are shown. The relative intensities of the protein bands (B-D,F-H) were quantified by ImageJ software. The data are expressed as the mean ± SD of the 3 independent experiments; #, P<0.05, ##, P<0.01, vs. control group; **, P<0.01 vs. RANKL group. HIF1α, hypoxia-inducible factor 1 alpha; RANKL, receptor activator of NF-kB ligand; L-MIM, L-mimosine; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, Jun N-terminal kinase; SD, standard deviation.