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Depletion of CD8+ (−CD8) or CD4+ (−CD4) T cells from splenocyte cultures of mice immunized three times with 40 μg of MAP4(PyCSP57–70) and 15 μg of Lipofectin. Effectors were stimulated with 2.5 μM PyCSP59–70. Lysis of target P815 cells coated with 2.5 μM PyCSP59–70 is shown. E:T Ratio, effector/target cell ratio.
