Figure 2.

Effect of length of homology on efficiency of targeted integration. (A) Disruption fragments used in this experiment. All five fragments have the same left and right ends, but the regions of homology extend for different lengths (from 35 to 55 bp) before joining with the URA3 gene, indicated by the number to the left of each fragment. The right-hand targeted regions of these disruption fragment are not shown, but these sequences also share the same end as one another and extend for the same lengths as the left hand ends. (B) The efficiency of transformation for disruption fragments of CAT8 (open circles) and RGT1 (filled circles) with targeted regions of different lengths as shown in (A). The error bars shown represent the standard error of the mean of three independent determinations, at least 60 transformants were screened for each determination.