Table 2. Efficiency of targeted replacement of URA3 gene.
Genotype | Percentage targeteda | |
---|---|---|
v:L:ub | v:L:U(199)c | |
CAT8:URA3 |
8 (100) |
19 (129) |
RGT2:URA3 |
7 (100) |
16 (118) |
RGT1:URA3 |
7 (96) |
19 (120) |
YHP1:URA3 |
7 (96) |
15 (120) |
SNF3:URA3 | 6 (98) | 17 (120) |
a(Replacements/total transformants) × 100%; numbers of transformants are in parentheses.
bv:L:u contains 40 bp of vector sequence present at the 5′ end of each disrupted gene, followed by LEU2, followed by 40 bp of URA3 sequence present at 3′ end of each disrupted gene.
cv:L:U(199) contains 40 bp of vector sequence present at the 5′ end of each disrupted gene, followed by LEU2, followed by 199 bp of URA3 sequence present at 3′ end of each disrupted gene.