Figure 4.

SF-1 is required for Tgfbr3l expression in adult murine pituitary glands. (A) Pituitary cDNA from control and cKO female and male mice (described in Fig. 3) were analyzed for Tgfbr3l expression by RT-qPCR. Data were analyzed by two-tailed unpaired t tests with Welch’s correlation. ***P < .001. Chromatin accessibility, as measured with single-nucleus ATAC-seq, over the (B) Tgfbr3l and (C) Cga genes in gonadotropes of 10- to 12-week-old control (orange) and cKO (grey) males. Exon 1 and the promoter of Tgfbr3l are boxed in red in B. Shown are representative tracks from a control and a cKO animal.