Table 1.
Primers
| Murine Tgfbr3l promoter amplification | |
| 5′ RACE gene-specific RT primer | GGACGGACGAGGTATTGTGA |
| 5′ RACE gene-specific outer R | CCTGCGTCCGGTATTCAATG |
| 5′ RACE gene-specific inner R | GGGCGTGAAGAAGGTGTTAC |
| −999/+1 promoter amplification F | AAAACTCGAGGTAGCTGATGCAACCATACGTAG |
| −999/+1 promoter amplification R | AAAAAAGCTTGACCGGCAGCGAGCACCT |
| Human TGFBR3L promoter amplification | |
| −996/+1 promoter amplification F | AAAACTCGAGTAGGCGTAGCATCCCTCTC |
| −996/+1 promoter amplification R | TTTTAAGCTTGGACCAGCTGAGGTCGGA |
| Tgfbr3l/TGFBR3L mutagenesis | |
| Tgfbr3l SF-1 site 1 mut F | ATCTGAGCACATGCTCAATTCCAGCCATGGATAAGGGC |
| Tgfbr3l SF-1 site 1 mut R | GCCCTTATCCATGGCTGGAATTGAGCATGTGCTCAGAT |
| Tgfbr3l SF-1 site 2 mut F | GGTGGCAGCCTCACCAATTCCAGGGCTACC |
| Tgfbr3l SF-1 site 2 mut R | GGTAGCCCTGGAATTGGTGAGGCTGCCACC |
| TGFBR3L SF-1 site 1 mut F | CCAGAGCCAATGCCCAATTCCTGAGGGGATTAAGGG |
| TGFBR3L SF-1 site 1 mut R | CCCTTAATCCCCTCAGGAATTGGGCATTGGCTCTGG |
| TGFBR3L SF-1 site 2 mut F | GGCGGCCCTGTAATTGGCAAGGAGGGAGGCA |
| TGFBR3L SF-1 site 2 mut R | TGCCTCCCTCCTTGCCAATTACAGGGCCGCC |
| qPCR primers | |
| Fshb F | GTGCGGGCTACTGCTACACT |
| Fshb R | CAGGCAATCTTACGGTCTCG |
| Gnrhr F | TTCGCTACCTCCTTTGTCGT |
| Gnrhr R | CACGGGTTTAGGAAAGCAAA |
| Lhb F | ACTGTGCCGGCCTGTCAACG |
| Lhb R | AGCAGCCGGCAGTACTCGGA |
| Nr5a1 F | AGGAGTTCGTCTGTCTCAAGTTCCT |
| Nr5a1 R | ACAAGGTGTAATCCAACAGGGCAG |
| Rpl19 F | CGGGAATCCAAGAAGATTGA |
| Rpl19 R | TTCAGCTTGTGGATGTGCTC |
| Tgfbr3l F | CCTGACACCAGTGCCTTTGA |
| Tgfbr3l R | CTAGGGGACGGACGAGGTAT |
| Tgfbr3l SF-1 ChIP qPCR F | TCAGTACATCAAGAAAGCCC |
| Tgfbr3l SF-1 ChIP qPCR R | GTACCCAGCCCTCTAGGT |
| Gene desert qPCR F | GTCACAGAAACGCAAAGGTTTA |
| Gene desert qPCR R | CCCAAAGTCATGTTGTACTTGATAG |
| Genotyping primers | |
| GRIC F | GGACATGTTCAGGGATCGCCAGGC |
| GRIC R | GCATAACCAGTGAAACAGCATTGCTG |
| Nr5a1 F | AGGAGTTCGTCTGTCTCAAGTTCC |
| Nr5a1 R | ACAAGGTGTAATCCAACAGGGCAG |
| Nr5a1 R (recombinant) | TGCGTGCAATCCATCTTGTTCAAT |