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. 2022 Dec 6;8(12):e12118. doi: 10.1016/j.heliyon.2022.e12118

Figure 3.

Figure 3

EphA2 depletion does not affect the transfection capacity of lipo2000. pEGFP-N plasmids (2.5 μg) were transfected into HCC shEphA2 and shNC cells using lipo2000. (A) The representative pictures of fluorescent GFP and Hoechst of HCC cells, Magnification 200×, scale bar 50μm. (B) Western blot indicating the level of GFP in HCC cells. SiNC (10 nm) and siGAPDH (10 nM) were transfected into HCC shEphA2 cells and shNC cells using lipo2000. (C) and (D) qPCR and Western blot indicating the level of EphA2 in HCC cells. ∗∗∗P < 0.001, “ns” means no significant difference.