FIGURE 2.

Proliferation of immune cells treated with YH29407 was significantly increased after stimulation with tumor-associated antigen (gp70-1, gp70-2). (A) MC38 tumor was grafted onto C57BL/6 mice and 5 days of treatment was started after the tumor volume reached 200 mm³. Then, splenocytes were obtained and cells were seeded after being counted using a hematocytometer, and the cells were stimulated with MC38 tumor lysate, gp70-1, gp70-2, ConA, and LPS for 72 h. Cell proliferation assay showed T cell (B) and innate immune activation (C). Moreover, tumor-specific T cells were enriched after stimulation with MC38 tumor lysate and tumor-associated antigen (D). *p < 0.05, **p ≤ 0.01, ****p ≤ 0.0001. ConA, concanavalin A; LPS, lipopolysaccharide; IFN-γ+, interferon gamma.