Fig. 2.

Proteomic analysis of FSA and surrounding PTT reveals strong expression differences between tissues. A) 3D-Principal Component Analysis plot of proteins detected in tumor and each PTT from 30 canine FSA. PCA was performed using all identified proteins. B-D) Volcano plots and Gene Set Enrichment analysis (GSEA) using KEGG pathways highlighting proteins that are differentially expressed between tumor and each PTT (red = significantly down in tumor, green = significantly up in tumor compared to respective PTT). Cutoff values for significance (p-value 0.05, log2(FC) = 1) are indicated using grey dashed lines. P-values were calculated using ANOVA; The volcano highlights proteins that are differently expressed between tumor tissue and B) connective tissue, C) adipose tissue, or D) skeletal muscle. GSEA for the individual comparisons are placed directly below the corresponding volcano plots.