Table 2.
Reagents and resources with Research Resource Identifiers (RRID) tags
| Reagent or resource | Source | Identifier | City & State | RRID | Dilution |
|---|---|---|---|---|---|
| Antibodies | |||||
| 6E10 beta-Amyloid 1–16(mouse monoclonal) | BioLegend | 803001 | San Diego, CA, USA | AB_2564653 | 1:500 |
| Anti-Abeta 40 (beta amyloid) (rabbit polyclonal) | MyBiosource | MBS555166 | San Diego, CA, USA | AB_2920623 | 1:500 |
| Anti-Abeta 42 (beta amyloid) (mouse monoclonal) | MyBiosource | MBS592140 | San Diego, CA, USA | AB_2920624 | 1:500 |
| Anti-Abeta 43 (beta amyloid) (mouse monoclonal) | BioLegend | 805601 | San Diego, CA, USA | AB_2564686 | 1:500 |
| Anti-beta amyloid [Pyro Glu3] (polyclonal) | Novus | NBP1-44048 | San Diego, CA, USA | AB_2056697 | 1:500 |
| Anti- beta amyloid mOC23 (rabbit monoclonal) | Abcam | ab205340 | Waltham,MA, USA | AB_2920639 | 1:500 |
| Anti- beta amyloid mOC31 (rabbit monoclonal) | Abcam | ab205340 | Waltham,MA, USA | AB_2920640 | 1:500 |
| Phospho-Tau (Ser202, Thr205) AT8 (mouse monoclonal) | Thermo fisher scientific | MN1020 | Waltham, MA, USA | AB_223647 | 1:500 |
| Human-Tau40 159PPGQK163HT7 (mouse monoclonal) | Thermo fisher scientific | MN1000 | Waltham, MA, USA | AB_223647 | 1:500 |
| Human Tau40 306VQIVYK311 amyloid motif (rabbit polyclonal) | ABclonal | A19560 | Woburn, MA, USA | AB_2862667 | 1:250 |
| Anti-PHF (Ser396, Ser404) | P. Davies. Albert Einstein College of Medicine | PHF1 | NY, USA | AB_2315150 | 1:500 |
| Anti-GFAP (chicken polyclonal) | Abcam | ab4674 | Waltham,MA, USA | AB_304558 | 1:1000 |
| Anti-Iba1 (rabbit polyclonal) | FUJIFILM WAKO | 019-19741 | Richmond, VA, USA | AB_839504 | 1:1000 |
| Anti-Parvalbumin (rabbit polyclonal) | Swant | PV-28 | Burgdorf, CHE | AB_2315235 | 1:1000 |
| Anti-NeuN (mouse monoclonal) | Abcam | ab104224 | Waltham,MA, USA | AB_10711040 | 1:1000 |
| Anti-cFos (rabbit polyclonal) | Abcam | ab190289 | Waltham,MA, USA | AB_2737414 | 1:500 |
| Anti-ubiquitin, P4D1 (mouse monoclonal) | Santa Cruz biotechnology | sc-8017 | Dallas, TX, USA | AB_628423 | 1:250 |
| Anti-α-smooth muscle actin (mouse monoclonal) | Santa Cruz biotechnology | sc-53,142 | Dallas, TX, USA | AB_2273670 | 1:250 |
| Cy3 Donkey anti mouse (secondary, polyclonal) | Jackson immuno research | 715-165-150 | West Grove, PA, USA | AB_2340813 | 1:200 |
| Cy5 Donkey anti mouse (secondary, polyclonal) | Jackson immuno research | 715-175-150 | West Grove, PA, USA | AB_2340819 | 1:200 |
| Cy3 Donkey anti rabbit (secondary, polyclonal) | Jackson immuno research | 711-165-152 | West Grove, PA, USA | AB_2307443 | 1:200 |
| Alexa Fluor 488 Donkey anti rabbit (secondary, polyclonal) | Jackson immuno research | 711-545-152 | West Grove, PA, USA | AB_2313584 | 1:200 |
| Cy3 Donkey anti Goat (secondary, polyclonal) | Jackson immuno research | 705-165-147 | West Grove, PA, USA | AB_2307351 | 1:200 |
| Alexa Fluor 488 Donkey anti Goat (secondary, polyclonal) | Jackson immuno research | 705-545-147 | West Grove, PA, USA | AB_2336933 | 1:200 |
| Alexa Fluor 488 Donkey anti Chicken (secondary, polyclonal) | Jackson immuno research | 703-545-155 | West Grove, PA, USA | AB_2340375 | 1:200 |
| Chemicals, stains and kits | |||||
| Amylo-Glo RTD | Biosensis | TR-300-AG | Thebarton, AUS | 1:100 | |
| Wisteria Floribunda Lectin/Agglutinin (WFA, WFL), Biotinylated | Vector laboratories | B-1355-2 | Newark, CA, USA | 1:1000 | |
| Abeta40 ELISA kit | MyBioSource | MBS760432 | San Diego, CA, USA | ||
| Abeta42 ELISA kit | MyBioSource | MBS726927 | San Diego, CA, USA | ||
| Alexa Fluor 488 Streptavidin | Jackson Immuno Research | 016-540-084 | West Grove, PA, USA | 1:500 | |
| TrueBlack lipofusion autofluorescence quencher | Biotium | 23007 | Fremont, CA | 1:20 | |