Figure 3.

Construction of T7D-rrnBP1 hybrid promoters and location of the footprint signals. Starting from the rrnBP1 promoter, sequence elements of T7D promoter were inserted at various positions so as to generate hybrid promoters, RF, RC, RFC and DR. Large grey arrows and brackets indicate the modified sequences. Signals obtained by DNase I and hydroxyl radical footprintings, shown in Figure 4, are indicated along these hybrid promoter sequences. Symbols are the same as in Figures 2 and 4. Guanines displaying increased reactivity against DMS in transcription complex (data not shown) are indicated by dots below the sequences.