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Specificity of the G. vaginalis 120-kDa Lbp. Equal amounts of protein from whole-cell lysates of G. vaginalis 594 (lanes 1) or G. vaginalis 317 (lanes 2) were electroblotted onto nitrocellulose filters. Prior to exposure to DIG-hLf, the filters were preincubated with various unlabeled iron-containing compounds. (A) no pretreatment; (B) hLf; (C) hemin; (D) hemoglobin; (E) catalase; (F) bovine lactoferrin; (G) human transferrin. The arrow indicates the 120-kDa G. vaginalis Lbp.
