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Sensitivity of the 120-kDa protein lactoferrin-binding activity to protease treatment. Western blot analysis was performed to examine the 120-kDa protein hLf-binding activity from G. vaginalis 594 cells treated with proteases prior to cell lysis. Lane 1, no protease treatment; lane 2, proteinase K treatment; lane 3, trypsin treatment. MW, molecular mass standards in kilodaltons.
