Fig. S3.
Cmpd-3 binding at the α1D-AR. Competition binding performed in membranes isolated from Rat-1 fibroblasts expressing the α1D-AR and using the radiolabeled antagonist, 125I-HEAT and in the presence of 0.5 mM GTP. Cmpd-3 (blue squares) binds with low affinity (Ki = 30 μM) at the α1D-AR. The orthosteric α1A-AR selective agonists, cirazoline (green triangles) and RO115-120 (pink diamonds) completely inhibited 125I-HEAT but with lower affinity at the α1D-AR. N = 4 independent experiments performed in duplicate. Data was analyzed in GraphPad Prism using the one-site competitive binding algorithm.