Fig. 10.

Cmpd-3 can potentiate the NE-mediated cAMP response in transfected Rat-1 fibroblasts (A), human SK-N-MC, a transformed neuroblastoma cell line (B) or primary mouse cardiomyocytes in a PAM assay (C). To show PAM effects of potency and Emax, the concentration of NE is held constant at its Emax (10⁻⁴ ​M) (red line). Cmpd-3 is then added in a dose-response (blue line). Any signal above the red line is potentiation. Cmpd-3 potentiates the NE-mediated cAMP response with a potency of 0.1 pM in neuroblastoma cells (A) or 0.5 ​nM in primary cardiomyocytes (B). Media alone (yellow squares). The shift to the right in the primary cells is due to endogenous receptor density. N ​= ​4(C); 6(B); 13(A) independent experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)