Figure 5. Cystinosin is required for ferroptosis suppression by extracellular albumin.
(A) Cell death in HT-1080N Control or cystinosin (CTNS) gene-disrupted (knockout, KO) cell lines. INK128: 1 µM, bovine serum albumin (BSA): 3% w/v. Data represent mean ± SD from three independent experiments. (B) C11 BODIPY 581/591 (C11) oxidation ratio (oxidized C11/[reduced C11 + oxidized C11]). INK128: 1 µM, BSA: 3% w/v. Data acquired from 59–512 individual cells per condition were analyzed. Representative data from one of three independent experiments is shown. (C) Total glutathione (GSH + GSSG) measured using Ellman’s reagent. INK128: 1 µM, BSA: 3% w/v. (D) Protein levels determined by western blot. C: Control, KO: knockout. Blots are representative of three independent experiments. See also Figure S6.