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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2022 Nov 26;733:109471. doi: 10.1016/j.abb.2022.109471

Table 2.

Calculated and Observed Masses for His-tagged NahE and Mutantsa

Enzyme Calculated Mass (Da) Observed Mass (Da)
WT 38,174.60 38,175.35
T65V 38,172.63 38,172.86
W128F 38,135.57 38,135.22
Y155F 38,158.61 38,157.94
N157L 38,173.66 38,173.65
N157A 38,131.58 38,130.18
N281L 38,173.66 38,173.98
a

Average molecular masses where standard deviations are presented in Table S3.