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. 2022 Oct 26;9(35):2204513. doi: 10.1002/advs.202204513

Figure 3.

Figure 3

Exosomal circATG4B promotes autophagy level. A) Western blotting showed that oxaliplatin‐resistant CRC cells induced the increased proportion of LC3B‐II/LC3B‐I. B,C) RAPA increased oxaliplatin resistance of CRC cells (B) while 3‐MA attenuated in chemoresistance of oxaliplatin‐resistant cells (C). D) 3‐MA reversed the chemoresistance induced by exosome‐control in sensitive cells. E) 3‐MA abolished circATG4B‐promoted oxaliplatin resistance in sensitive cells. F) Puncta‐like staining detection of CRC cells transfected with various treatments is shown. Scale bars, 10 µm. G) Transmission electron microscopy analysis of autophagy is shown. Arrows: autophagosomes/autolysosomes. Scale bars, 2 µm. H) LC3B‐II accumulation and p62 of CRC cells transfected with various treatments is shown. I) IHC analysis of Ki67, LC3B, and p62 in subcutaneous tumor. Scale bars, 20 µm.