TABLE 2.
MAbs obtained from the LDA Workshop: reactivity with the surface of bone marrow cells and effect on adhesion of gametocyte-infected erythrocytesa
| Antibody | Clone | Type | Specificityb | Characteristicsc
|
|||
|---|---|---|---|---|---|---|---|
| L88/5
|
trHBMEC
|
||||||
| Effect on adhesion | Detection on surface | Effect on adhesion | Detection on surface | ||||
| A002 | ASC-1 | Mouse IgG1 | CD49c | ++ | + | + | + |
| A004 | ASC-2 | Mouse IgG1 | NK | − | − | − | + |
| A006 | ASC-5 | Mouse IgG1 | CD49c | − | + | − | + |
| A007 | ASC-6 | Mouse IgG1 | CD49c | − | + | − | + |
| A008 | ASC-8 | Mouse IgG1 | CD104 | − | − | − | + |
| A009 | ASC-9 | Mouse IgG2a | CD104 | − | − | − | + |
| A010 | ASC-10 | Mouse IgG1 | CD49c | − | + | − | + |
| A013 | Lia1/2 | Mouse IgG1 | CD29 | − | + | − | + |
| A025 | F430C-5 | Mouse IgG2a | CD105 | − | + | − | + |
| A029 | J3-119 | Mouse IgG2a | CD166 | − | + | − | + |
| A030 | J4-81 | Mouse IgG2a | CD166 | +++ | + | ++ | + |
| A047 | MEM63 | Mouse IgG1 | CD58 | − | + | − | + |
| A091 | VC5 | Mouse IgG1 | CD49e | − | + | − | + |
| A097 | B-D12 | Mouse IgG1 | CD71 | − | + | − | + |
| A101 | 4A11 | Mouse IgM | NK | − | − | − | + |
| A102 | ASC-11 | Mouse IgG1 | NK | − | − | − | + |
| A105 | CJ1250 | Mouse IgM | NK | − | + | − | + |
| A107 | CNA42 | Mouse IgM | NK | − | − | − | + |
| A108 | AD2 | Mouse IgG1 | CD165 | − | + | − | + |
| A109 | 3A6 | Mouse IgG1 | CD166 | − | + | − | + |
| A111 | F4-35H7 | Mouse IgG1 | CD146 | − | + | − | + |
| A112 | F432G3 | Mouse IgG1 | CD146 | − | + | − | + |
| A113 | F439E | Mouse IgG1 | CD146 | − | + | − | + |
| A115 | 105A5 | Mouse IgM | CD164 | ++ | − | + | − |
| A117 | J1-G3B | Mouse IgG1 | NK | − | + | − | + |
| A119 | J3-E1B | Mouse IgG1 | CD98 | − | + | − | + |
| A120 | H8E3 | Mouse IgG3 | NK | − | + | − | + |
| A124 | 9E10 | Mouse IgG3 | CD164 | − | − | + | + |
| A126 | RMcB | Mouse IgG2b | NK | − | − | − | + |
a
All antibody data are from the LDA website (http://www.mh-hannover.de/projekte/hlda7/hldabase/select.htm).
b
NK, not known.
c
+, 15 to 30% inhibition of adhesion/positive reaction with cell surface; ++, 30 to 50% inhibition of adhesion; +++, >50% inhibition of adhesion; −, no effect on adhesion/not detected on cell surface.