Skip to main content
. Author manuscript; available in PMC: 2022 Dec 19.
Published in final edited form as: SLAS Discov. 2022 Oct 7;27(8):448–459. doi: 10.1016/j.slasd.2022.09.005

Fig. 1. Optimization of cell-based assay expressing FLAG-CRFBP(10kD)-HA-CRF2α using the [35S]GTPγS binding assay.

Fig. 1.

(A) CRF produced a dose dependent stimulation of [35S]GTPγS-binding FLAG-CRF-BP(10kD)-HA-CRF2α. (B) AS-30 inhibits CRF-stimulated (1 μM) [35S]GTPγS FLAG-CRF-BP(10kD)-HA-CRF2α binding. The values are expressed as M ± SEM percentage increase in basal [35S]GTPγS binding.