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. Author manuscript; available in PMC: 2022 Dec 19.
Published in final edited form as: SLAS Discov. 2022 Oct 7;27(8):448–459. doi: 10.1016/j.slasd.2022.09.005

Fig. 8. CRFBP-CRF2 modulators do not affect CRF1 activity on action potential dependent GABA transmission in the CeA.

Fig. 8.

A) Electrophysiological recordings were performed from CRF1+ labeled neurons in CeA slices. B) Representative traces of mIPSCs at baseline, in the presence of R121219 (1 μM) and MLS-0046818 (30 μM), and R121219 (1 μM) + MLS-0046818 (30 μM) + CRF (200 nM). C) Application of R121219 (1 μM) and MLS-0046818 (30 μM) for 15 min does not induce significant changes: one sample t-test, p = 0.5575 (frequency), p = 0.0908 (amplitude), p = 0.2017 (rise time) and p = 0.4208 (decay) in mIPSC properties relative to baseline (one-sample t-test; n = 9 neurons/6 mice). D) Application of CRF (200 nM) following pre-treatment of brain slices with R121219 (1 μM) and MLS-0046818 (30 μM) for 15 min do not produce significant changes: one sample t-test, p = 0.8948 (frequency), p = 0.3619 (amplitude), p = 0.7754 (rise time) and p = 0.1513 (decay) in mIPSC properties relative to baseline (one-sample t-test, n = 8 neurons/4 mice).