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. 2022 Dec 19;17(12):e0270317. doi: 10.1371/journal.pone.0270317

Fig 2. Oprm1 mRNA and 3HDAMGO binding is not altered in the Oprm1-Cre mouse line compared to wild type C57Bl/6 mice.

Fig 2

Animals expressing the T2A-Cre allele showed no significant molecular differences of Oprm1-expression when compared to wild-type littermates. (A) Quantification of the qPCR analysis of Oprm1 between Oprm1Cre/+ and Oprm1+/+ animals shows that there is comparable Oprm1 mRNA transcript levels in the hippocampus, hypothalamus, PFC, cerebellum, striatum, and thalamus, regardless of genotype (Males, 10-15wks old, n = 6). Two-way ANOVA revealed no main effect of genotype between groups (F (1,60) = 0.5438, p = 0.4637). (B) 3H-DAMGO binding is not different between groups. (Males, 10-15wks old, n = 6–7). Two-way ANOVA revealed a main interaction effect (F(2, 32) = 3.306, p = 0.0495. Post hoc test revealed no difference between Oprm1Cre/+ and Oprm1+/+ mouse lines in any brain region.