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. 2022 Dec 19;17(12):e0277863. doi: 10.1371/journal.pone.0277863

Fig 4. Hypoxic- (HCM) and normoxic- (NCM) hASCs secretomes accelerate in vitro cell migration and show in vitro and in vivo pro-angiogenic potential.

Fig 4

(A) Representative image of the migration of HUVEC cells after 0 or 18 h of incubation in HCM, NCM or control. Quantification of the percentage of variation in the wound area (% migration area). Scale bars = 250 μm (B) Representative images of EC-coated beads with NCM or HCM on fibrin gel and stained with calcein. Quantitation of EC sprouting formation and sprout length in arbitrary units formed after 4 days. Scale bars = 100μm (C) Mixture of matrigel-filled plugs containing indicated conditioned medium (NCM or HCM) or PBS (control) were injected subcutaneously in mice (n = 8). After 11 days, hemoglobin content representative of invading vessels was measured. The two-way ANOVA test and the Bonferroni post-test were used to analyze the differences among the groups. Values are expressed as mean ± SD of at least three independent experiments. (C) **p<0.01; ***p<0.001 (n = 12, 6 per group).