Skip to main content
. 2022 Dec 19;17(12):e0277863. doi: 10.1371/journal.pone.0277863

Fig 5. NG2+Nestin+ pericytes surrounding the blood vessels and in close contact with the vascular wall in hASCs secretomes treated animals.

Fig 5

(A) Representative macroscopic images showing cutaneous wounds on days 0 and 7 after injection of control, hypoxic- (HCM) or normoxic- (NCM) secretomes. (B) HCM and NCM accelerated wound closure and microvessel density. Confocal images of the skin wound sections labeled NG2+(red)/Nestin+(green) pericytes and CD31+ blood vessels (blue). Use of pseudocolor (white) to display colocalization of NG2+(red) and nestin+(green) pericytes around CD31+microvessels (blue). (C) The extent of microvessel density was determined by assessing the CD31+ vessel area or NG2+nestin+ area in each of 4 randomly chosen high-power fields within the injury site. Scale bar, 100 μm for images in (B). Results are given as the means ± the SD. *p<0.05, **p<0.01; ***p<0.001 (n = 12, 6 per group).